Journal article
Whole-cell phase contrast imaging at the nanoscale using Fresnel Coherent Diffractive Imaging Tomography
MWM Jones, GA Van Riessen, B Abbey, CT Putkunz, MD Junker, E Balaur, DJ Vine, I McNulty, B Chen, BD Arhatari, S Frankland, KA Nugent, L Tilley, AG Peele
Scientific Reports | NATURE PORTFOLIO | Published : 2013
DOI: 10.1038/srep02288
Abstract
X-ray tomography can provide structural information of whole cells in close to their native state. Radiation-induced damage, however, imposes a practical limit to image resolution, and as such, a choice between damage, image contrast, and image resolution must be made. New coherent diffractive imaging techniques, such Fresnel Coherent Diffractive Imaging (FCDI), allows quantitative phase information with exceptional dose efficiency, high contrast, and nano-scale resolution. Here we present three-dimensional quantitative images of a whole eukaryotic cell by FCDI at a spatial resolution below 70 nm with sufficient phase contrast to distinguish major cellular components. From our data, we estim..
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Funding Acknowledgements
The authors acknowledge support from the Australian Research Council Centre of Excellence for Coherent X-ray Science. We acknowledge travel funding provided by the International Synchrotron Access Program (ISAP) managed by the Australian Synchrotron and funded by the Australian Government. Use of the Advanced Photon Source is supported by the U.S. Department of Energy, Office of Science, and Office of Basic Energy Sciences, under Contract No. DE-AC02-06CH11357.