The metabolic framework of pluripotent stem cells and potential mechanisms of regulation

Abstract

It is becoming increasingly clear that standard measures of embryonic stem cell pluripotency do not necessarily reflect underlying differences in cell physiology. There is an immediate need to expand our knowledge of the basic physiology of pluripotent stem cells and to understand the impact of factors that regulate pluripotent stem-cell expansion, maintenance, and controlled differentiation on cell metabolism. Importantly, the responses of pluripotent stem cells to alterations in culture environment have not been extensively elucidated; this is especially relevant with the recent growth in specialized media formulations available. Coincident with the development and selection of media for p..