Journal article
Caveolin-1 is necessary for hepatic oxidative lipid metabolism: Evidence for crosstalk between caveolin-1 and bile acid signaling
MA Fernández-Rojo, M Gongora, RL Fitzsimmons, N Martel, SD Martin, SJ Nixon, AJ Brooks, MP Ikonomopoulou, S Martin, HP Lo, SA Myers, C Restall, C Ferguson, PF Pilch, SL McGee, RL Anderson, MJ Waters, JF Hancock, SM Grimmond, GEO Muscat Show all
Cell Reports | CELL PRESS | Published : 2013
Abstract
Caveolae and caveolin-1 (CAV1) have been linked to several cellular functions. However, a model explaining their roles in mammalian tissues invivo is lacking. Unbiased expression profiling in several tissues and cell types identified lipid metabolism as the main target affected by CAV1 deficiency. CAV1-/- mice exhibited impaired hepatic peroxisome proliferator-activated receptor α (PPARα)-dependent oxidative fatty acid metabolism and ketogenesis. Similar results were recapitulated in CAV1-deficient AML12 hepatocytes, suggesting at least a partial cell-autonomous role of hepatocyte CAV1 in metabolic adaptation to fasting. Finally, our experiments suggest that the hepatic phenotypes observed i..
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Funding Acknowledgements
This work was supported by grants from the National Health and Medical Research Council (NHMRC) of Australia (to R.G.P., G.E.O.M., S.G., and R.L.A.) and from the National Breast Cancer Foundation (R.L.A.). M.A.F.-R. was supported by the Program of MEC/Fulbright postdoctoral fellowships from the Spanish government, the Diabetes Australia Research Trust, and the NHMRC. We thank all the members of Parton laboratory for support and discussion. The authors acknowledge the facilities as well as scientific and technical assistance from the staff in the Australian Cancer Research Foundation, the Institute for Molecular Bioscience Dynamic Imaging Facility for Cancer Biology, and the Australian Microscopy and Microanalysis Facility at the Centre for Microscopy and Microanalysis at the University of Queensland.