Journal article

Chemical characterisation and speciation of organic selenium in cultivated selenium-enriched Agaricus bisporus

Tebo Maseko, Damien L Callahan, Frank R Dunshea, Augustine Doronila, Spas D Kolev, Ken Ng

FOOD CHEMISTRY | ELSEVIER SCI LTD | Published : 2013

Abstract

The selenium concentration in Agaricus bisporus cultivated in growth compost irrigated with sodium selenite solution increased by 28- and 43-fold compared to the control mushroom irrigated solely with water. Selenium contents of mushroom proteins increased from 13.8 to 60.1 and 14.1 to 137 μgSe/g in caps and stalks from control and selenised mushrooms, respectively. Selenocystine (SeCys; detected as [SeCys]2 dimer), selenomethionine (SeMet), and methyl-selenocysteine (MeSeCys) were separated, identified and quantified by liquid chromatography-electrospray ionisation-mass spectrometry from water solubilised and acetone precipitated proteins, and significant increases were observed for the sel..

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