Mechanistic Insights into the 1,3-Xylanases: Useful Enzymes for Manipulation of Algal Biomass
Ethan D Goddard-Borger, Keishi Sakaguchi, Stephan Reitinger, Nobuhisa Watanabe, Makoto Ito, Stephen G Withers
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | AMER CHEMICAL SOC | Published : 2012
Xylanases capable of degrading the crystalline microfibrils of 1,3-xylan that reinforce the cell walls of some red and siphonous green algae have not been well studied, yet they could prove to be of great utility in algaculture for the production of food and renewable chemical feedstocks. To gain a better mechanistic understanding of these enzymes, a suite of reagents was synthesized and evaluated as substrates and inhibitors of an endo-1,3-xylanase. With these reagents, a retaining mechanism was confirmed for the xylanase, its catalytic nucleophile identified, and the existence of -3 to +2 substrate-binding subsites demonstrated. Protein crystal X-ray diffraction methods provided a high res..View full abstract
E.D.G.B thanks the Canadian Institutes of Health Research for a postdoctoral fellowship. S.R. thanks the Austrian Science Fund for an Erwin Schrodinger Fellowship. S.G.W. acknowledges support from the Natural Sciences and Engineering Research Council of Canada, the Canada Research Chairs Program, the Canadian Foundation for Innovation, and the B.C. Knowledge Development Fund. We thank Shouming He of the Proteomics Core Facility at UBC for mass spectrometric analyses.