Journal article
In vitro differentiation of near-unlimited numbers of functional mouse basophils using conditional Hoxb8
U Gurzeler, T Rabachini, CA Dahinden, M Salmanidis, G Brumatti, PG Ekert, N Echeverry, D Bachmann, HU Simon, T Kaufmann
Allergy European Journal of Allergy and Clinical Immunology | Published : 2013
DOI: 10.1111/all.12140
Abstract
Background Basophils constitute a rare leukocyte population known for their effector functions in inflammation and allergy, as well as more recently described immunoregulatory roles. Besides their low frequency, functional analysis of basophils is hindered by a short life span, inefficient ex vivo differentiation protocols, and lack of suitable cell models. A method to produce large quantities of basophils in vitro would facilitate basophil research and constitute a sought-after tool for diagnostic and drug testing purposes. Methods A method is described to massively expand bone marrow-derived basophils in vitro. Myeloid progenitors are conditionally immortalized using Hoxb8 in the presence ..
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Awarded by Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
Funding Acknowledgements
We thank Barbara Geering for discussions and critical comments on the manuscript; Andrea Kaufmann and the FACS sorting facility of the Department of Clinical Research, University of Bern, for expert technical assistance; John Silke (Melbourne, AU) for the lentiviral inducible system; and Andreas Strasser, David Huang, Lorraine O'Reilly (all Melbourne, AU), Gunnar Nilsson (Stockholm, SE), Georg Hacker (Munich, DE), Christoph Borner (Freiburg, DE), Shida Yousefi, and Nadia Corazza (all Bern, CH), for mice, antibodies, and reagents. This study was supported by grants to TK form the Swiss National Science Foundation (PP0033_119203) and the 3R Research Foundation Switzerland (3R-127-11).