Journal article

Expression and purification of recombinant annexin V for the detection of membrane alterations on apoptotic cells

Gabriela Brumatti, Clare Sheridan, Seamus J Martin

METHODS | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2008

Abstract

Apoptosis is a mode of cell death that is accompanied by specific alterations to the plasma membrane that promote the recognition and engulfment of these cells by phagocytes. Although several such membrane alterations have been defined, redistribution of phosphatidylserine from the inner to the outer plasma membrane leaflet has become one of the most widely used markers for apoptotic cells in mammals. This is largely due to the availability of a sensitive and specific probe for this event in the form of the phosphatidylserine-binding protein, annexin V. Here, we describe methods for the expression and purification of recombinant polyhistidine-tagged annexin V from Escherichia coli. Recombina..

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