Journal article
Oryza Tag Line, a phenotypic mutant database for the Génoplante rice insertion line library
P Larmande, C Gay, M Lorieux, C Périn, M Bouniol, G Droc, C Sallaud, P Perez, I Barnola, C Biderre-petit, J Martin, JB Morel, AAT Johnson, F Bourgis, A Ghesquière, M Ruiz, B Courtois, E Guiderdoni
Nucleic Acids Research | Published : 2008
DOI: 10.1093/nar/gkm762
Open access
Abstract
To organize data resulting from the phenotypic characterization of a library of 30 000 T-DNA enhancer trap (ET) insertion lines of rice ( Oryza sativa L cv. Nipponbare), we developed the Oryza Tag Line (OTL) database http://urgi.versailles.inra.fr/OryzaTagLine OTL structure facilitates forward genetic search for specific phenotypes, putatively resulting from gene disruption, and/or for GUSA or GFP reporter gene expression patterns, reflecting ET-mediated endogenous gene detection. In the latest version, OTL gathers the detailed morpho-physiological alterations observed during field evaluation and specific screens in a first set of 13 928 lines. Detection of GUS or GFP activity in specific or..
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