Journal article

A Fully Human Inhibitory Monoclonal Antibody to the Wnt Receptor RYK

Michael M Halford, Maria L Macheda, Clare L Parish, Elena A Takano, Stephen Fox, Daniel Layton, Edouard Nice, Steven A Stacker

PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2013

Abstract

RYK is an unusual member of the receptor tyrosine kinase (RTK) family that is classified as a putative pseudokinase. RYK regulates fundamental biological processes including cell differentiation, migration and target selection, axon outgrowth and pathfinding by transducing signals across the plasma membrane in response to the high affinity binding of Wnt family ligands to its extracellular Wnt inhibitory factor (WIF) domain. Here we report the generation and initial characterization of a fully human inhibitory monoclonal antibody to the human RYK WIF domain. From a naïve human single chain fragment variable (scFv) phage display library, we identified anti-RYK WIF domain-specific scFvs then s..

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Grants

Funding Acknowledgements

This work was funded partly by a Program Grant from the National Health and Medical Research Council of Australia (NHMRC) to SAS and an NHMRC Project Grant to CLP. SAS is supported by a Senior Research Fellowship from the NHMRC. CLP is supported by a Viertel Senior Medical Research Fellowship, Australia. SAS would like to acknowledge the support of the Pfizer Australia Fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.