Journal article

Cyclosporine and Tacrolimus Have Inhibitory Effects on Toll-Like Receptor Signaling After Liver Transplantation

Jessica Howell, Rohit Sawhney, Adam Testro, Narelle Skinner, Paul Gow, Peter Angus, Dilip Ratnam, Kumar Visvanathan



Toll-like receptors (TLRs) play a key role in transplantation biology. The effect of immunosuppression on TLR function after liver transplantation is unknown. Peripheral blood mononuclear cells (PBMCs) from 113 post-liver transplant patients and 13 healthy controls were stimulated with TLR-specific ligands [lipopolysaccharide (TLR4), pan-3-cys (P3C) (TLR2), Poly (I:C) (PIC) (TLR3), R848 (TLR7/8), and CpG (TLR9)] for 24 hours. PBMCs from 5 healthy controls were also cultured with therapeutic concentrations of cyclosporine A (CYA) and tacrolimus (TAC). Cytokine production was measured with enzyme-linked immunosorbent assays and flow cytometry. PBMCs from patients on calcineurin inhibitors afte..

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Funding Acknowledgements

Funding for this study was provided by nonspecific Innate Immune Laboratory educational funds, which are supported by Monash University (Melbourne Australia). Jessica Howell received scholarship funds for a stipend from the Gastroenterological Society of Australia.