Journal article
Effect of probiotic bacteria on the intestinal microbiota in irritable bowel syndrome
SC Ng, EFC Lam, TTY Lam, Y Chan, W Law, PCH Tse, MA Kamm, JJY Sung, FKL Chan, JCY Wu
Journal of Gastroenterology and Hepatology Australia | Published : 2013
DOI: 10.1111/jgh.12306
Abstract
Background and Aim: In irritable bowel syndrome (IBS), the gut microbiota may be altered. Probiotic bacteria appear to be therapeutically effective. We characterized the mucosa-associated microbiota, and determined the clinical and microbiological effects of orally administered probiotic bacteria, in patients with IBS. Methods: Mucosal microbiota from rectal biopsies of IBS patients and controls were assessed on the V1 and V2 variable regions of the 16S ribosomal RNA gene amplified using 454 pyrosequencing. Clinical symptoms and changes in mucosal microbiota were assessed in IBS patients before and after 4 weeks of treatment with probiotic mix VSL#3. Results: Ten IBS subjects (eight female; ..
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Funding Acknowledgements
We would like to acknowledge Patrick Law at the Chinese University of Hong Kong for performing the sequencing experiments, YK Tse for statistical input, and Li Ka Shing Institute of Health Sciences where part of the work was performed. This project was supported by the Faculty of Medicine Direct Grant. TT Lam was supported by Newton International Fellowship from Royal Society, UK. Ferring Pharmaceuticals, Hong Kong is the source of VSL#3 and they have no input into the study design, manuscript content or decision to publish.