Yeast hEST1A/B (SMG5/6)- Like proteins contribute to environment-sensing adaptive gene expression responses

Abstract

During its natural life cycle, budding yeast (Saccharomyces cerevisiae) has to adapt to drastically changing environments, but how environmental-sensing pathways are linked to adaptive gene expression changes remains incompletely understood. Here, we describe two closely related yeast hEST1A-B (SMG5-6)-like proteins termed Esl1 and Esl2 that contain a 14-3-3-like domain and a putative PilT Nterminus ribonuclease domain. We found that, unlike their metazoan orthologs, Esl1 and Esl2 were not involved in nonsense-mediated mRNA decay or telomere maintenance pathways. However, in genomewide expression array analyses, absence of Esl1 and Esl2 led to more than two-fold deregulation of ~50 transcrip..