Condensin I associates with structural and gene regulatory regions in vertebrate chromosomes
Ji Hun Kim, Tao Zhang, Nicholas C Wong, Nadia Davidson, Jovana Maksimovic, Alicia Oshlack, William C Earnshaw, Paul Kalitsis, Damien F Hudson
NATURE COMMUNICATIONS | NATURE PUBLISHING GROUP | Published : 2013
The condensin complex is essential for correct packaging and segregation of chromosomes during mitosis and meiosis in all eukaryotes. To date, the genome-wide location and the nature of condensin-binding sites have remained elusive in vertebrates. Here we report the genome-wide map of condensin I in chicken DT40 cells. Unexpectedly, we find that condensin I binds predominantly to promoter sequences in mitotic cells. We also find a striking enrichment at both centromeres and telomeres, highlighting the importance of the complex in chromosome segregation. Taken together, the results show that condensin I is largely absent from heterochromatic regions. This map of the condensin I binding sites ..View full abstract
Awarded by Australian Research Council
Awarded by National Health and Medical Research Council (NHMRC)
Awarded by Wellcome Trust
This work was supported by an Australian Research Council discovery project (grant number DP110100784, National Health and Medical Research Council (NHMRC) project grants (APP1047009) and by the Victorian Government's Operational Infrastructure Support Program. W. C. E. is a Principal Research Fellow of the Wellcome Trust (grant number 073915). A.O. is a NHMRC Career Development Fellow. We thank K. H. Andy Choo, Jeffrey Mann, and Kathryn Marshall for helpful comments and discussion of the manuscript.