Journal article

Characterisation of macaque testicular leucocyte populations and T-lymphocyte immunity

Robert De Rose, Caroline S Fernandez, Mark P Hedger, Stephen J Kent, Wendy R Winnall

JOURNAL OF REPRODUCTIVE IMMUNOLOGY | ELSEVIER IRELAND LTD | Published : 2013

Abstract

The rodent testis is well established as a site of immune privilege where both innate and acquired immune responses are suppressed. Immune cells and responses within human or non-human primate testes, by contrast, are poorly characterised. This study used multi-colour flow cytometry to characterise the leukocytes in testicular cells isolated from 12 young adult pigtail macaques (Macaca nemestrina) by collagenase dispersal, and to measure the cytokine responses of macaque testicular T-lymphocytes to mitogens. B-lymphocytes and granulocytes were present in very low numbers (0.24% and 3.3% of leukocytes respectively), indicating minimal blood contamination. A median of 30.8% of the recovered te..

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Grants

Awarded by NHMRC fellowships


Awarded by NHMRC programme grant


Funding Acknowledgements

We thank Sinthujan Jegaskanda, Thakshila Amarasena, Sheilajen Alcantara, Sarah Lloyd, Matthew Parsons and Gayle Davey for expert technical assistance, providing reagents and/or for assistance with manuscript preparation. This work was supported by NHMRC fellowships (1013221, 1041832, 1020269 and 1011578), the NHMRC programme grant 510448 and the Victorian Government's Operational Infrastructure Support Programme (to Monash Institute of Medical Research).