Bacteriophages in Gut Samples From Pediatric Crohn's Disease Patients: Metagenomic Analysis Using 454 Pyrosequencing
Josef Wagner, Jovana Maksimovic, Gabriella Farries, Winnie H Sim, Ruth F Bishop, Donald J Cameron, Anthony G Catto-Smith, Carl D Kirkwood
INFLAMMATORY BOWEL DISEASES | OXFORD UNIV PRESS INC | Published : 2013
BACKGROUND: The role of bacteriophage in Crohn's disease (CD) is unknown. This study investigated the abundance of phages in ileal and colonic samples from pediatric CD patients and controls. METHODS: Ileal and colonic biopsies from 6 CD patients, gut wash samples from 3 CD patients, and ileal biopsies from 6 noninflammatory bowel disease patients (controls) were analyzed for the presence of bacteriophage using 454 high-throughput pyrosequencing. A sequence-independent single-primer amplification method was used to amplify viral sequences. RESULTS: A total of 186,143 high quality reads were obtained from the 4 sample populations. Contigs and sequence clusters (generated from unassembled sing..View full abstract
Awarded by National Health and Medical Research Council (NHMRC)
Awarded by NHMRC RD Wright Research Fellowship
Supported by (1) National Health and Medical Research Council (NHMRC) research grants (607382), (2) Theme Funding from the Murdoch Childrens Research Insitute, (3) Victorian Government's Operational Infrastructure Support Program, and (4) Dr C. D. Kirkwood is supported by an NHMRC RD Wright Research Fellowship (607347).