Journal article

A Low-Cost, Small Volume Circuit for Autologous Blood Normothermic Perfusion of Rabbit Organs

Murray Worner, Samuel Poore, Daniel Tilkorn, Zerina Lokmic, Anthony J Penington

Artificial Organs | WILEY | Published : 2014

Abstract

We have designed a laboratory extracorporeal normothermic blood perfusion system for whole organs (e.g., kidney) that achieves pulsatile flow, low levels of hemolysis, and a blood priming volume of 60 mL or less. Using this uniquely designed extracorporeal circuit, we have achieved perfusion of two isolated ex vivo constructs. In the first experiment, we successfully perfused a rabbit epigastric flap based on the femoral vessels. In the second experiment, we were able to perfuse the isolated rabbit kidney for 48 h (range for all kidneys was 12-48 h) with excellent urine output, normal arterial blood gasses at 24 h, and normal ex vivo kidney histology at the conclusion of the experiments. The..

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