Pharmacological priming of adipose-derived stem cells for paracrine VEGF production with deferoxamine
Guei-Sheung Liu, Hitesh M Peshavariya, Masayoshi Higuchi, Elsa C Chan, Gregory J Dusting, Fan Jiang
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE | WILEY | Published : 2016
Adipose-derived stem cells (ASCs) show great potentials in applications such as therapeutic angiogenesis, regenerative medicine and tissue engineering. Pharmacological preconditioning of stem cells to boost the release of cytoprotective factors may represent an effective way to enhance their therapeutic efficacy. In this study, the aim was to determine whether deferoxamine can enhance the release of vascular endothelial growth factor (VEGF) from in vitro expanded ASCs. It is demonstrated that deferoxamine (50-300 μm) upregulated VEGF expression in a concentration- and time-dependent fashion. At the concentrations used, deferoxamine did not show any cytotoxic effects. The stimulatory effect o..View full abstract
Awarded by National Health and Medical Research Council of Australia
Awarded by Natural Science Foundation of China
The authors thank Nancy Guo for providing expert technical assistance. This work was partly supported by research grants from the National Health and Medical Research Council of Australia (NHMRC 09007G) (G.L.), the JO&JR Wicking Trust, he Ansell Ophthalmology Foundation and Natural Science Foundation of China (81070164) (F.J.). G.J.D. is supported by a Principal Research Fellowship from NHMRC. The Centre for Eye Research Australia and the O'Brien Institute acknowledge the Victorian State Government's Department of Innovation, Industry and Regional Development's Operational Infrastructure Support Programme.