Journal article
Insights into the immuno-molecular biology of Angiostrongylus vasorum through transcriptomics-Prospects for new interventions
BRE Ansell, M Schnyder, P Deplazes, PK Korhonen, ND Young, RS Hall, S Mangiola, PR Boag, A Hofmann, PW Sternberg, AR Jex, RB Gasser
Biotechnology Advances | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2013
Abstract
Angiostrongylus vasorum is a metastrongyloid nematode of dogs and other canids of major clinical importance in many countries. In order to gain first insights into the molecular biology of this worm, we conducted the first large-scale exploration of its transcriptome, and predicted essential molecules linked to metabolic and biological processes as well as host immune responses. We also predicted and prioritized drug targets and drug candidates. Following Illumina sequencing (RNA-seq), 52.3. million sequence reads representing adult A. vasorum were assembled and annotated. The assembly yielded 20,033 contigs, which encoded proteins with 11,505 homologues in Caenorhabditis elegans, and additi..
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Awarded by National Institutes of Health
Funding Acknowledgements
This project was funded by the Australian Research Council and National Health and Medical Research Council (NHMRC) (R.B.G.), Howard Hughes Medical Institute (HHMI) and the National Institutes of Health (NIH) (P.W.S.). This project was also supported by a Victorian Life Sciences Computation Initiative (VLSCI) grant number VR0007 on its Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government. Other support from the Australian Academy of Science, the Australian-American Fulbright Commission, Alexander von Humboldt Foundation, Melbourne Water Corporation and the IBM Research Collaboratory for Life Sciences-Melbourne is gratefully acknowledged (R.B.G.). N.D.Y. is an NHMRC Early Career Research (ECR) Fellow. We also acknowledge the continued contributions of all staff at WormBase (www.wormbase.org).