Journal article
Distinct nuclear receptor expression in stroma adjacent to breast tumors
KC Knower, AL Chand, N Eriksson, K Takagi, Y Miki, H Sasano, JE Visvader, GJ Lindeman, JW Funder, PJ Fuller, ER Simpson, WD Tilley, PJ Leedman, JD Graham, GEO Muscat, CL Clarke, CD Clyne
Breast Cancer Research and Treatment | SPRINGER | Published : 2013
Abstract
The interaction between breast tumor epithelial and stromal cells is vital for initial and recurrent tumor growth. While breast cancer-associated stromal cells provide a favorable environment for proliferation and metastasis, the molecular mechanisms contributing to this process are not fully understood. Nuclear receptors (NRs) are intracellular transcription factors that directly regulate gene expression. Little is known about the status of NRs in cancer-associated stroma. Nuclear Receptor Low-Density Taqman Arrays were used to compare the gene expression profiles of all 48 NR family members in a collection of primary cultured cancer-associated fibroblasts (CAFs) obtained from estrogen rece..
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Awarded by U.S. Department of Defense
Funding Acknowledgements
This study was supported by the National Health and Medical Research Council of Australia through fellowships to CDC (#338518), JEV (Australia Fellow), and GJL (637307), ERS, PJF, and CLC; and NHMRC IRIISS; GEOM is the University of Queensland Vice Chancellors research fellowship; the Victorian State Government through Victoria Cancer Agency funding of the Victoria Breast Cancer Research Consortium and the Victorian Government's Operational Infrastructure Support Program; the United States Department of Defense fellowship to ALC; a Collaborative Program from the National Breast Cancer Foundation Australia. Breast cancer and normal tissues were provided by Australian Breast Cancer Tissue Bank (ABCTB), which is supported by the National Health and Medical Research Council of Australia, the Cancer Institute NSW, and the National Breast Cancer Foundation, or by the Victorian Cancer BioBank Australia, which is supported by the Victorian Government. ABCTB tissues and samples were made available to researchers on a nonexclusive basis. Normal breast biopsies were obtained from the Susan G. Komen for the Cure Tissue Bank at the IU Simon Cancer Center. The authors thank contributors to the Susan G. Komen for the Cure Tissue Bank, including Indiana University that collected the samples used in this study, as well as parents and families whose participation and help made this study possible. The authors would like to thank Drs Elgene Lim and Francois Vaillant for their assistance in stromal cell collection, and Silke Kantimm for assistance with immunohistochemistry. PHI Data Audit #13-08.