Immunopurification of adenomatous polyposis coli (APC) proteins.
Kerryn L Elliott, Bruno Catimel, Nicole L Church, Janine L Coates, Antony W Burgess, Meredith J Layton, Maree C Faux
BMC Res Notes | Published : 2013
BACKGROUND: The adenomatous polyposis coli (APC) tumour suppressor gene encodes a 2843 residue (310 kDa) protein. APC is a multifunctional protein involved in the regulation of β-catenin/Wnt signalling, cytoskeletal dynamics and cell adhesion. APC mutations occur in most colorectal cancers and typically result in truncation of the C-terminal half of the protein. RESULTS: In order to investigate the biophysical properties of APC, we have generated a set of monoclonal antibodies which enable purification of recombinant forms of APC. Here we describe the characterisation of these anti-APC monoclonal antibodies (APC-NT) that specifically recognise endogenous APC both in solution and in fixed cel..View full abstract