Clinical isolates and laboratory reference Candida species and strains have varying abilities to form biofilms
Ali D Alnuaimi, Neil M O'Brien-Simpson, Eric C Reynolds, Michael J McCullough
FEMS YEAST RESEARCH | OXFORD UNIV PRESS | Published : 2013
Candida biofilms are a major virulence trait for this yeast. In this study, the biofilm-forming ability of the major medically important clinical and laboratory reference strains was compared. Biofilms were quantified using traditional methods, that is, crystal violet (CV), tetrazolium (XTT) reduction and colony-forming unit assays (CFU), and two new methods: an automated cell counter (ACC) and biofilm suspension turbidity (BST) method. Biofilms could be categorized based on biofilm biomass (high, medium and low) and growth state (high and low). Candida albicans genotypes, A, B and C, showed medium biofilm mass and low growth rate, and only one C. albicans laboratory strain, ATCC MYA-2719, m..View full abstract
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Awarded by National Health and Medical Research Council (NHMRC)
This work was supported by the National Health and Medical Research Council (NHMRC), research Grant Number: APP1029878.