Journal article
Postnatal shifts in ischemic tolerance and cell survival signaling in murine myocardium
NY Liaw, L See Hoe, FL Sheeran, JN Peart, JP Headrick, MMH Cheung, S Pepe
American Journal of Physiology Regulatory Integrative and Comparative Physiology | AMER PHYSIOLOGICAL SOC | Published : 2013
Abstract
The immature heart is known to be resistant to ischemia-reperfusion (I/R) injury; however, key proteins engaged in phospho-dependent signaling pathways crucial to cell survival are not yet defined. Our goal was to determine the postnatal changes in myocardial tolerance to I/R, including baseline expression of key proteins governing I/R tolerance and their phosphorylation during I/R. Hearts from male C57Bl/6 mice (neonates, 2, 4, 8, and 12 wk of age, n = 6/group) were assayed for survival signaling/effectors [Akt, p38MAPK, glycogen synthase kinase-3β (GSK-3ββ), heat shock protein 27 (HSP27), connexin-43, hypoxia-inducible factor-1α (HIF-1α), and caveolin-3] and regulators of apoptosis (Bax an..
View full abstractGrants
Awarded by Australian National Health and Medical Research Council
Awarded by Royal Children's Hospital Foundation
Funding Acknowledgements
This study was funded by an Australian National Health and Medical Research Council Project Grant (481922). Studies conducted at the Murdoch Childrens Research Institute were also supported by the Victorian Government's Operational Infrastructure Support Program and the Royal Children's Hospital Foundation (RCH1000).