Journal article
Isolation of human lymphatic malformation endothelial cells, their in vitro characterization and in vivo survival in a mouse xenograft model
Z Lokmic, GM Mitchell, N Koh Wee Chong, J Bastiaanse, YW Gerrand, Y Zeng, ED Williams, AJ Penington
Angiogenesis | SPRINGER | Published : 2014
Abstract
Abstract: Human lymphatic vascular malformations (LMs), also known as cystic hygromas or lymphangioma, consist of multiple lymphatic endothelial cell-lined lymph-containing cysts. No animal model of this disease exists. To develop a mouse xenograft model of human LM, CD34NegCD31 Pos LM lymphatic endothelial cells (LM-LEC) were isolated from surgical specimens and compared to foreskin CD34NegCD31Pos lymphatic endothelial cells (LECs). Cells were implanted into a mouse tissue engineering model for 1, 2 and 4 weeks. In vitro LM-LECs showed increased proliferation and survival under starvation conditions (P < 0.0005 at 48 h, two-way ANOVA), increased migration (P < 0.001, two-way ANOVA) and form..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
We thank Michelle Marchionna for technical assistance in the early stages of this project. This work was supported by grants from The National Health and Medical Research Council (NHMRC #566886), The Hector Waldron Pride Charitable Trust (EDW, AJP) and The William Buckland Foundation (AJP, MM). EDW was supported by an NHMRC Career Development Award (#519539). The O'Brien Institute and Monash Institute of Medical Research acknowledge support from the Victorian State Government's Department of Innovation, Industry and Regional Development's Operational Infrastructure Support Program.