Journal article

The inhibitory effect of proanthocyanidin on soluble and collagen-bound proteases

Don Jeevanie Epasinghe, Cynthia Kar Yung Yiu, Michael Francis Burrow, Noriko Hiraishi, Franklin Russell Tay

JOURNAL OF DENTISTRY | ELSEVIER SCI LTD | Published : 2013

Abstract

OBJECTIVE: This study evaluated the inhibitory effect of proanthocyanidin (PA), a natural collagen cross-linker, on soluble and matrix-bound proteases, which are responsible for progressive degradation of exposed collagen fibrils within the hybrid layer and resin-dentine bond failure over time. METHODS: The inhibitory effects of PA (1%, 2%, 3%, 4.5% and 6%) on soluble recombinant matrix metalloproteinases (MMP-2, -8 and -9) and cysteine cathepsins (cathepsin B and K) were evaluated using MMP and cysteine cathepsins fluorometric assay kits. Chlorhexidine (CHX) was used as an inhibitor control. The effect of PA on endogenous matrix-bound proteases was examined by determining the change in dry ..

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