The inhibitory effect of proanthocyanidin on soluble and collagen-bound proteases
Don Jeevanie Epasinghe, Cynthia Kar Yung Yiu, Michael Francis Burrow, Noriko Hiraishi, Franklin Russell Tay
JOURNAL OF DENTISTRY | ELSEVIER SCI LTD | Published : 2013
OBJECTIVE: This study evaluated the inhibitory effect of proanthocyanidin (PA), a natural collagen cross-linker, on soluble and matrix-bound proteases, which are responsible for progressive degradation of exposed collagen fibrils within the hybrid layer and resin-dentine bond failure over time. METHODS: The inhibitory effects of PA (1%, 2%, 3%, 4.5% and 6%) on soluble recombinant matrix metalloproteinases (MMP-2, -8 and -9) and cysteine cathepsins (cathepsin B and K) were evaluated using MMP and cysteine cathepsins fluorometric assay kits. Chlorhexidine (CHX) was used as an inhibitor control. The effect of PA on endogenous matrix-bound proteases was examined by determining the change in dry ..View full abstract
Awarded by Faculty of Dentistry, The University of Hong Kong
This work was supported by Grant 201011159189, Faculty of Dentistry, The University of Hong Kong.