Journal article
Phagocytosis of neonatal pathogens by peripheral blood neutrophils and monocytes from newborn preterm and term infants
A Prosser, J Hibbert, T Strunk, CH Kok, K Simmer, P Richmond, D Burgner, A Currie
Pediatric Research | Published : 2013
DOI: 10.1038/pr.2013.145
Abstract
Background:Deficiencies in phagocytosis may contribute to the increased susceptibility of infants to early life infections. Data on phagocytosis of the major neonatal pathogens Staphylococcus epidermidis (SE), Staphylococcus aureus (SA), and Escherichia coli (EC) by preterm infant leukocytes are inconsistent.Methods:Cord and <24-h peripheral blood were collected from very preterm (<30.1 wks gestational age (GA)) and term (37-42 wks GA) infants. Monocyte and neutrophil phagocytosis of pHrodo-labeled SE, SA, and EC were analyzed using a small-volume flow cytometry assay, with simultaneous characterization of surface activation marker expression.Results:Preterm infants had lower proportions of ..
View full abstractGrants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
This study was supported by Australian National Health and Medical Research Council project grants 513847 and 572548. Andrew Currie is supported by a fellowship from the BrightSpark Foundation of Western Australia. David Burgner is supported by a National Health and Medical Research Council Career Development Fellowship and the Victorian Government's Operational Infrastructure Support Program.