Journal article
Overexpression of carnitine palmitoyltransferase-1 in skeletal muscle is sufficient to enhance fatty acid oxidation and improve high-fat diet-induced insulin resistance
CR Bruce, AJ Hoy, N Turner, MJ Watt, TL Allen, K Carpenter, GJ Cooney, MA Febbraio, EW Kraegen
Diabetes | AMER DIABETES ASSOC | Published : 2009
DOI: 10.2337/db08-1078
Abstract
OBJECTIVE-Skeletal muscle insulin resistance is associated with lipid accumulation, but whether insulin resistance is due to reduced or enhanced flux of long-chain fatty acids into the mitochondria is both controversial and unclear. We hypothesized that skeletal muscle-specific overexpression of the muscle iso- form of carnitine palmitoyltransferase 1 (CPT1), the enzyme that controls the entry of long-chain fatty acyl CoA into mitochondria, would enhance rates of fatty acid oxidation and improve insulin action in muscle in high-fat diet insulin-resistant rats. RESEARCH DESIGN AND METHODS-Rats were fed a standard (chow) or high-fat diet for " weeks. After 3 weeks, in vivo electrotransfer was ..
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Funding Acknowledgements
This work was supported by research grants from the Diabetes Australia Research Trust. C.R.B. has received a NHMRC Peter Doherty Postdoctoral fellowship. A.J.H. has received a University of New South Wales Postgraduate Award. N.T. has received a NHMRC Career Development Award. M.J.W. has received a NHMRC R Douglas Wright Fellowship. G.J.C. is a Senior Research Fellow of the NHMRC. M.A.F. is a Principal Research Fellow of the NHMRC. E.W.K. is a Senior Principal Research Fellow of the NHMRC.No potential conflicts of interest relevant to this article were reported.