Journal article
The genome and developmental transcriptome of the strongylid nematode Haemonchus contortus
EM Schwarz, PK Korhonen, BE Campbell, ND Young, AR Jex, A Jabbar, RS Hall, A Mondal, AC Howe, J Pell, A Hofmann, PR Boag, XQ Zhu, TR Gregory, A Loukas, BA Williams, I Antoshechkin, CT Brown, PW Sternberg, RB Gasser
Genome Biology | Published : 2013
Open access
Abstract
Background: The barber's pole worm, Haemonchus contortus, is one of the most economically important parasites of small ruminants worldwide. Although this parasite can be controlled using anthelmintic drugs, resistance against most drugs in common use has become a widespread problem. We provide a draft of the genome and the transcriptomes of all key developmental stages of H. contortus to support biological and biotechnological research areas of this and related parasites.Results: The draft genome of H. contortus is 320 Mb in size and encodes 23,610 protein-coding genes. On a fundamental level, we elucidate transcriptional alterations taking place throughout the life cycle, characterize the p..
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Awarded by National Institutes of Health
Funding Acknowledgements
This paper is dedicated to the memories of Sue Newton and Paul J Presidente. We thank Sujai Kumar and Mark Blaxter for providing methods for removing contaminant sequences, and Ali Mortazavi for early sequence analyses. We are grateful to Jody Zawadzki and the late Paul Presidente for originally providing us with the McMaster and Haecon-5 strains of H. contortus. This project was funded by the Australian Research Council (RBG and PWS), National Health and Medical Research Council (NHMRC), Howard Hughes Medical Institute, and the National Institutes of Health. This project was also supported by a Victorian Life Sciences Computation Initiative (grant number VR0007) on its Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government. Other support from the Australian Academy of Science, the Australian-American Fulbright Commission, Alexander von Humboldt Foundation, Melbourne Water Corporation, and the IBM Research Collaboratory for Life Sciences Melbourne is gratefully acknowledged. NDY is an NHMRC Early Career Research Fellow. We also acknowledge the contributions of all staff at WormBase. We thank the anonymous reviewers for their very constructive reports on our original manuscript.