Journal article
Circulating precursor CCR7loPD-1hi CXCR5 CD4 T cells indicate tfh cell activity and promote antibody responses upon antigen reexposure
J He, LM Tsai, YA Leong, X Hu, CS Ma, N Chevalier, X Sun, K Vandenberg, S Rockman, Y Ding, L Zhu, W Wei, C Wang, A Karnowski, GT Belz, JR Ghali, MC Cook, DS Riminton, A Veillette, PL Schwartzberg Show all
Immunity | Published : 2013
Abstract
Follicular B helper T (Tfh) cells support high affinity and long-term antibody responses. Here we found that within circulating CXCR5+ CD4+ Tcells in humans and mice, the CCR7loPD-1hi subset has a partial Tfh effector phenotype, whereas CCR7hiPD-1lo cells have a resting phenotype. The circulating CCR7loPD-1hi subset was indicative of active Tfh differentiation in lymphoid organs and correlated with clinical indices in autoimmune diseases. Thus the CCR7loPD-1hi subset provides a biomarker to monitor protective antibody responses during infection or vaccination and pathogenic antibody responses in autoimmune diseases. Differentiation of both CCR7hiPD-1lo and CCR7loPD-1hi subsets required ICOS ..
View full abstractGrants
Awarded by National Human Genome Research Institute
Funding Acknowledgements
We thank FlowCore, Monash University, and Garvan MLC Flow Cytometry Facility for cell sorting and providing analytical instruments, Monash and Garvan animal facilities for animal husbandry and technical services, Karen Laurie and Anne Kelso for suggestion on influenza vaccination, Andrew Wei for suggestion on immune response after hematopoietic stem cell transplantation, and Zdenka Prodanovic (the Tissue Bank Southern Health) for collecting human tissue samples. This work was funded by National Basic Research Program of China (973 Program, 2010CB529100) To Z.L. and D.Y. National Health and Medical Research Council (NHMRC, 1010761), Australian Research Council (DP110105024), Ramaciotti Foundation and National Natural Science Foundation of China (NSFC, 81128012) to D.Y., Beijing Natural Science Foundation (7131015) to Z.L. NSFC (3107088) to J.H., and NHMRC program to C. R. M., C. G. V., S. G. T., R. B., and F. M. D.Y. is supported by a NHMRC Fellowship (GNT1011947) and a Monash Fellowship. C. S. M. is supported by a NHMRC Fellowship. C. G. V. is supported by a Viertel Senior Medical Research Fellowship. C. R. M. is supported by a NHMRC Australian Fellowship.