Journal article
Protein Kinase Activity of Phosphoinositide 3-Kinase Regulates Cytokine-Dependent Cell Survival
D Thomas, JA Powell, BD Green, EF Barry, Y Ma, J Woodcock, S Fitter, ACW Zannettino, SM Pitson, TP Hughes, AF Lopez, PR Shepherd, AH Wei, PG Ekert, MA Guthridge
Plos Biology | Published : 2013
Abstract
The dual specificity protein/lipid kinase, phosphoinositide 3-kinase (PI3K), promotes growth factor-mediated cell survival and is frequently deregulated in cancer. However, in contrast to canonical lipid-kinase functions, the role of PI3K protein kinase activity in regulating cell survival is unknown. We have employed a novel approach to purify and pharmacologically profile protein kinases from primary human acute myeloid leukemia (AML) cells that phosphorylate serine residues in the cytoplasmic portion of cytokine receptors to promote hemopoietic cell survival. We have isolated a kinase activity that is able to directly phosphorylate Ser585 in the cytoplasmic domain of the interleukin 3 (IL..
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Funding Acknowledgements
[ "This work was supported by NH&MRC Australia, the Association of International Cancer Research UK, the Leukaemia Foundation of Australia, and the Peter Nelson Foundation. D.T. is a recipient of a Haematology & Oncology Targeted Therapy (HOTT) Fellowship administered by Haematology Society of Australia & New Zealand, and P.E. is a Sylvia and Charles Viertel Fellow. Organizations providing financial support for the present work had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.", "These studies were supported by funding from the National Health and Medical Research Council (NHMRC) of Australia, Leukaemia Foundation of Australia, Victorian Cancer Agency, and the Alfred Hospital Foundation. We thank L. Foukas (University College London), C. Mitchell (Monash University), and Shaun Jackson (Australian Centre for Blood Diseases) for valuable reagents and cells." ]