Targeted-capture massively-parallel sequencing enables robust detection of clinically informative mutations from formalin-fixed tumours
Stephen Q Wong, Jason Li, Renato Salemi, Karen E Sheppard, Hongdo Do, Richard W Tothill, Grant A McArthur, Alexander Dobrovic
Scientific Reports | NATURE PUBLISHING GROUP | Published : 2013
This project was enabled by the Peter MacCallum Pfizer Translational Oncology Research Collaborative Hub (TORCH) funded by Pfizer Australia, and the Melbourne Melanoma Project funded by the Victorian Cancer Agency Translational research program and established through support of the Victor Smorgon Charitable Fund. We thank Carleen Cullinane for preparing the xenograft blocks and Jia-Min Pang for review of slides for contaminating normal mouse tissue in xenografted formalin-fixed sections. We also thank Andrew Fellowes, Anthony Bell, and Stephen Fox for the TruSeq reagent and access to the MiSeq instrument.