Journal article

Differential Roles of the Protein Corona in the Cellular Uptake of Nanoporous Polymer Particles by Monocyte and Macrophage Cell Lines

Yan Yan, Katelyn T Gause, Marloes MJ Kamphuis, Ching-Seng Ang, Neil M O'Brien-Simpson, Jason C Lenzo, Eric C Reynolds, Edouard C Nice, Frank Caruso

ACS NANO | AMER CHEMICAL SOC | Published : 2013

Abstract

Many biomolecules, mainly proteins, adsorb onto polymer particles to form a dynamic protein corona in biological environments. The protein corona can significantly influence particle-cell interactions, including internalization and pathway activation. In this work, we demonstrate the differential roles of a given protein corona formed in cell culture media in particle uptake by monocytes and macrophages. By exposing disulfide-stabilized poly(methacrylic acid) nanoporous polymer particles (PMASH NPPs) to complete cell growth media containing 10% fetal bovine serum, a protein corona, with the most abundant component being bovine serum albumin, was characterized. Upon adsorption onto the PMASH ..

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Grants

Awarded by Australian Research Council under the Australian Laureate Fellowship


Awarded by Discovery Early Career Researcher Award


Awarded by National Health and Medical Research Council (NHMRC) Program


Funding Acknowledgements

This work was supported by the Australian Research Council under the Australian Laureate Fellowship (F.C., FL120100030) and Discovery Early Career Researcher Award (Y.Y, DE130100488) and by the National Health and Medical Research Council (NHMRC) Program Grant 487922 (F.C.). The authors gratefully acknowledge Dr. Jiwei Cui (The University of Melbourne) for assistance with the particle synthesis and Ms. Shane Reeve (Monash Biomedical Proteomics Facility, Monash University, Clayton) for the initial mass spectrometry analysis on the HCT ULTRA ion trap mass spectrometer. Dr. Yee-Foong Mok (Macromolecular Interactions Facility Platform, Bio21 Institute, The University of Melbourne) is acknowledged for assistance with the circular dichroism analysis.