Journal article

Optical Imaging of Human Cone Photoreceptors Directly Following the Capture of Light

Phillip Bedggood, Andrew Metha



Capture of light in the photoreceptor outer segment initiates a cascade of chemical events that inhibit neurotransmitter release, ultimately resulting in vision. The massed response of the photoreceptor population can be measured non-invasively by electrical recordings, but responses from individual cells cannot be measured without dissecting the retina. Here we used optical imaging to observe individual human cones in the living eye as they underwent bleaching of photopigment and associated phototransduction. The retina was simultaneously stimulated and observed with high intensity visible light at 1 kHz, using adaptive optics. There was marked variability between individual cones in both p..

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Awarded by Australian Research Council

Funding Acknowledgements

This project was supported by an Australian Research Council Discovery Early Career Researcher Award (DE120101931), an Australia Research Council Discovery Project Grant (DP0984649), an A.E. Rowden White Foundation benevolent bequest, and the University of Melbourne Interdisciplinary Seed Fund. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.