Cryptosporidium parvum genotype IIa and Giardia duodenalis assemblage A in Mytilus galloprovincialis on sale at local food markets
Annunziata Giangaspero, Roberto Papini, Marianna Marangi, Anson V Koehler, Robin B Gasser
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | ELSEVIER | Published : 2014
To date, there has been no study to establish the genotypic or subgenotypic identities of Cryptosporidium and Giardia in edible shellfish. Here, we explored the genetic composition of these protists in Mytilus galloprovincialis (Mediterranean mussel) purchased from three markets in the city of Foggia, Italy, from May to December 2012. Samples from the digestive glands, gills and haemolymph were tested by nested PCR, targeting DNA regions within the 60 kDa glycoprotein (gp60) gene of Cryptosporidium, and the triose-phosphate isomerase (tpi) and β-giardin genes of Giardia. In total, Cryptosporidium and Giardia were detected in 66.7% of mussels (M. galloprovincialis) tested. Cryptosporidium was..View full abstract
The authors wish to thank Antonio Narducci and Raffaella Terlizzi for their laboratory assistance and Professor Giovanni Normanno for the helpful discussions on food inspection. RBG's laboratory is currently funded by the Australian Research Council (ARC) and the National Health and Medical Research Council (NHMRC). Other support from Melbourne Water Corporation and the Alexander von Humboldt Foundation is gratefully acknowledged (RBG).