Journal article
Ex-vivo whole blood secretion of interferon (IFN)-γ and IFN-γ-inducible protein-10 measured by enzyme-linked immunosorbent assay are as sensitive as IFN-γ enzyme-linked immunospot for the detection of gluten-reactive T cells in human leucocyte antigen (HLA)-DQ2·5 -associated coeliac disease
N Ontiveros, JA Tye-Din, MY Hardy, RP Anderson
Clinical and Experimental Immunology | Published : 2014
DOI: 10.1111/cei.12232
Abstract
Summary: T cell cytokine release assays are used to diagnose infectious diseases, but not autoimmune or allergic disease. Coeliac disease (CD) is a common T cell-mediated disease diagnosed by the presence of gluten-dependent intestinal inflammation and serology. Many patients cannot be diagnosed with CD because they reduce dietary gluten before medical workup. Oral gluten challenge in CD patients treated with gluten-free diet (GFD) mobilizes gluten-reactive T cells measurable by interferon (IFN)-γ enzyme-linked immunospot (ELISPOT) or major histocompatibility complex (MHC) class II tetramers. Immunodominant peptides are quite consistent in the 90% of patients who possess HLA-DQ2·5. We aimed ..
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Awarded by NHMRC Independent Research Institutes Infrastructure Support Scheme
Funding Acknowledgements
The authors wish to thank the volunteers who participated in this study, the assistance of Dr Jessica Biesiekierski and the support of Coeliac Victoria and Tasmania in volunteer recruitment, and Mr Adam Girardin for technical assistance. N. O. was supported by a Mexican Scholarship by the Mexican Council for Science and Technology (CONACyT); J. T.-D. was supported by an NHMRC Postgraduate Medical Scholarship; M. H. was supported by an NHMRC Project Grant; and R. P. A. held the Ian Mackay Fellowship from the Walter and Eliza Hall Institute and Melbourne Health. The work was supported by Coeliac Australia, NHMRC Project Grant number 406656, the NHMRC Independent Research Institutes Infrastructure Support Scheme grant no. 361646 and Victorian State Government Operational Infrastructure Support.