Host Pathways Important for Coxiella burnetii Infection Revealed by Genome-Wide RNA Interference Screening
Justin A McDonough, Hayley J Newton, Scott Klum, Rachel Swiss, Herve Agaisse, Craig R Roy
MBIO | AMER SOC MICROBIOLOGY | Published : 2013
UNLABELLED: Coxiella burnetii is an intracellular pathogen that replicates within a lysosome-like vacuole. A Dot/Icm type IVB secretion system is used by C. burnetii to translocate effector proteins into the host cytosol that likely modulate host factor function. To identify host determinants required for C. burnetii intracellular growth, a genome-wide screen was performed using gene silencing by small interfering RNA (siRNA). Replication of C. burnetii was measured by immunofluorescence microscopy in siRNA-transfected HeLa cells. Newly identified host factors included components of the retromer complex, which mediates cargo cycling between the endocytic pathway and the Golgi apparatus. Redu..View full abstract
Awarded by Ruth L. Kirschstein National Research Service Award Individual Postdoctoral Fellowship
Awarded by NIH
Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
We thank Isabelle Derre and members of the Roy laboratory for helpful advice. This research was supported by a Ruth L. Kirschstein National Research Service Award Individual Postdoctoral Fellowship (F32-AI0829272) to J.A.M. and by NIH grants (R01-AI064559 and Northeast Biodefense Center U54-AI057158-Lipkin) to C. R. R.