Journal article

Host Pathways Important for Coxiella burnetii Infection Revealed by Genome-Wide RNA Interference Screening

Justin A McDonough, Hayley J Newton, Scott Klum, Rachel Swiss, Herve Agaisse, Craig R Roy

MBIO | AMER SOC MICROBIOLOGY | Published : 2013

Abstract

UNLABELLED: Coxiella burnetii is an intracellular pathogen that replicates within a lysosome-like vacuole. A Dot/Icm type IVB secretion system is used by C. burnetii to translocate effector proteins into the host cytosol that likely modulate host factor function. To identify host determinants required for C. burnetii intracellular growth, a genome-wide screen was performed using gene silencing by small interfering RNA (siRNA). Replication of C. burnetii was measured by immunofluorescence microscopy in siRNA-transfected HeLa cells. Newly identified host factors included components of the retromer complex, which mediates cargo cycling between the endocytic pathway and the Golgi apparatus. Redu..

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University of Melbourne Researchers

Grants

Awarded by Ruth L. Kirschstein National Research Service Award Individual Postdoctoral Fellowship


Awarded by NIH


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

We thank Isabelle Derre and members of the Roy laboratory for helpful advice. This research was supported by a Ruth L. Kirschstein National Research Service Award Individual Postdoctoral Fellowship (F32-AI0829272) to J.A.M. and by NIH grants (R01-AI064559 and Northeast Biodefense Center U54-AI057158-Lipkin) to C. R. R.