Journal article
Visualization of mouse barrel cortex using ex-vivo track density imaging
ND Kurniawan, KL Richards, Z Yang, D She, JFP Ullmann, RX Moldrich, S Liu, JU Yaksic, G Leanage, I Kharatishvili, V Wimmer, F Calamante, GJ Galloway, S Petrou, DC Reutens
Neuroimage | Published : 2014
Abstract
We describe the visualization of the barrel cortex of the primary somatosensory area (S1) of ex vivo adult mouse brain with short-tracks track density imaging (stTDI). stTDI produced much higher definition of barrel structures than conventional fractional anisotropy (FA), directionally-encoded color FA maps, spin-echo T1- and T2-weighted imaging and gradient echo T1/T2*-weighted imaging. 3D high angular resolution diffusion imaging (HARDI) data were acquired at 48micron isotropic resolution for a (3mm)3 block of cortex containing the barrel field and reconstructed using stTDI at 10micron isotropic resolution. HARDI data were also acquired at 100micron isotropic resolution to image the whole ..
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Funding Acknowledgements
We are grateful to the National Health and Medical Research Council (NHMRC) of Australia and the Australian Research Council (ARC) for their support. The Florey Institute of Neuroscience and Mental Health is supported by Victorian State Government infrastructure funds. We thank the Queensland State Government support for the operation of the 16.4 T through the Queensland NMR Network. JUY was supported by the Chilean National Scholarship. We thank Prof. IJ Richards for critical reading of the manuscript and histology.