Journal article

The Macrophage Galactose-Type Lectin Can Function as an Attachment and Entry Receptor for Influenza Virus

Wy Ching Ng, Stella Liong, Michelle D Tate, Tatsuro Irimura, Kaori Denda-Nagai, Andrew G Brooks, Sarah L Londrigan, Patrick C Reading



Specific protein receptors that mediate internalization and entry of influenza A virus (IAV) have not been identified for any cell type. Sialic acid (SIA), the primary attachment factor for IAV hemagglutinin, is expressed by numerous cell surface glycoproteins and glycolipids, confounding efforts to identify specific receptors involved in virus infection. Lec1 Chinese hamster ovary (CHO) epithelial cells express cell surface SIA and bind IAV yet are largely resistant to infection. Here, we demonstrate that expression of the murine macrophage galactose-type lectin 1 (MGL1) by Lec1 cells enhanced Ca(2+)-dependent IAV binding and restored permissivity to infection. Lec1 cells expressing MGL1 we..

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Awarded by National Health and Medical Research Council (NHMRC) of Australia

Awarded by Grants-in-Aid for Scientific Research

Funding Acknowledgements

[ "This study was supported by project grant 1027545 from the National Health and Medical Research Council (NHMRC) of Australia. P.C.R., S.L.L., and A.G.B. are all recipients of funding from the NHMRC. W.N. is a recipient of an NHMRC Dora Lush Biomedical Research Scholarship. S.L.L. is a recipient of a University of Melbourne Early Career Research Grant and a University of Melbourne, Melbourne Research Fellowship.", "The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health." ]