Detection of activated platelets in a mouse model of carotid artery thrombosis with 18F-labeled single-chain antibodies

K Ardipradja; SD Yeoh; K Alt; G O'Keefe; A Rigopoulos; DW Howells; AM Scott; K Peter; U Ackerman; CE Hagemeyer

Journal article

Detection of activated platelets in a mouse model of carotid artery thrombosis with 18F-labeled single-chain antibodies

K Ardipradja, SD Yeoh, K Alt, G O'Keefe, A Rigopoulos, DW Howells, AM Scott, K Peter, U Ackerman, CE Hagemeyer

Nuclear Medicine and Biology | Published : 2014

DOI: 10.1016/j.nucmedbio.2013.12.006

Abstract

Introduction: Activated platelets are key players in thrombosis and inflammation. We previously generated single-chain antibodies (scFv) against ligand-induced binding sites (LIBS) on the highly abundant platelet glycoprotein integrin receptor IIb/IIIa. The aim of this study was the construction and characterisation of a novel 18F PET radiotracer based on this antibody. Methods: ScFvanti-LIBS and control antibody mut-scFv were reacted with N-succinimidyl-4-[18F]fluorobenzoate (S[18F]FB). Radiolabeled scFv was incubated with in vitro formed platelet clots and injected into mice with FeCl3 induced thrombus in the left carotid artery. Clots were imaged in the PET scanner and amount of radioacti..

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University of Melbourne Researchers

Graeme O'Keefe Author

Andrew Scott Author

Karlheinz Peter Author

Uwe Ackermann Author

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Grants

Awarded by Australian Research Council

Funding Acknowledgements

This work was funded by the National Health and Medical Research Council (NHMRC), Grants 1029249, 1017670 and 1011418. K.Ar. is supported by the NHMRC and the National Heart Foundation (586740). K.Al. is supported by the German Research Foundation (AL 1521/1-1). K.P. is supported by an Australian Research Council Future Fellowship (FT0992210). C.E.H. is supported by a NHMRC Fellowship (472668). The work was also supported in part by the Victorian Government's Operational Infrastructure Support Program.