Journal article
The primary microRNA-208b interacts with Polycomb-group protein, Ezh2, to regulate gene expression in the heart
P Mathiyalagan, J Okabe, L Chang, Y Su, XJ Du, A El-Osta
Nucleic Acids Research | OXFORD UNIV PRESS | Published : 2014
DOI: 10.1093/nar/gkt896
Open access
Abstract
The Polycomb-group protein, Ezh2, is required for epigenetic gene silencing in the adult heart by unknown mechanism. We investigated the role of Ezh2 and non-coding RNAs in a mouse model of pressure overload using transverse aortic constriction (TAC) attenuated by the prototypical histone deacetylase inhibitor, trichostatin A (TSA). Chromatin immunoprecipitation of TAC and TAC+TSA hearts suggests interaction of Ezh2 and primary microRNA-208b (pri-miR-208b) in the regulation of hypertrophic gene expression. RNAi silencing of pri-miR-208b and Ezh2 validate pri-miR-208b-mediated transcriptional silencing of genes implicated in cardiac hypertrophy including the suppression of the bi-directional ..
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Funding Acknowledgements
The Victorian Government's Operational Infrastructure Support program (in part). Funding for open access charge: National Health and Medical Research Council and National Heart Foundation of Australia.