Journal article
Reproducible selection of high avidity CD8 T-cell clones following secondary acute virus infection
T Cukalac, J Chadderton, A Handel, PC Doherty, SJ Turner, PG Thomas, NL La Gruta
Proceedings of the National Academy of Sciences of the United States of America | NATL ACAD SCIENCES | Published : 2014
Abstract
The recall of memory CD8+ cytotoxic T lymphocytes (CTLs), elicited by prior virus infection or vaccination, is critical for immune protection. The extent to which this arises as a consequence of stochastic clonal expansion vs. active selection of particular clones remains unclear. Using a parallel adoptive transfer protocol in combination with single cell analysis to define the complementarity determining region (CDR) 3α and CDR3β regions of individual Tcell receptor (TCR) heterodimers, we characterized the antigendriven recall of the same memory CTL population in three individual recipients. This high-resolution analysis showed reproducible enrichment (or diminution) of particular TCR clono..
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Awarded by National Institute of Allergy and Infectious Diseases
Funding Acknowledgements
This work was supported by National Health and Medical Research Council (NHMRC) Project Grants AI628316 and AI1046333 (to N.L.L.G.), National Institute of Health Grants AI091938 (to P.G.T. and A.H.) and AI107625 (to P.G.T.), a Sylvia and Charles Viertel Senior Medical Research fellowship (to N.L.L.G.), an NHMRC Biomedical Postgraduate Scholarship ID520643 (to T.C.), and an Australian Research Council Future Fellowship (to S.J.T.).