Nucleic Acids: Hybridisation

Abstract

Abstract Nucleic acid hybridisation is the pairing of complementary deoxyribonucleic acid (DNA) strands to produce DNA–DNA hybrids or the pairing of complementary DNA–RNA strands to produce DNA–RNA hybrids. Nucleic acid hybridisation is the basis of many research and diagnostic applications with all relying on simple base pairing specificity of nucleic acids to generate a signal through a labelled probe. This fundamental principle has led to DNA/RNA detection and quantification on solid phase blots, DNA/RNA cytogenetic localisation on cells, detection and purification of specific DNA and comparative gene expressio..