Journal article
The unconventional role of acid sphingomyelinase in regulation of retinal microangiopathy in diabetic human and animal models
M Opreanu, M Tikhonenko, S Bozack, TA Lydic, GE Reid, KM McSorley, A Sochacki, GI Perez, WJ Esselman, T Kern, R Kolesnick, MB Grant, JV Busik
Diabetes | AMER DIABETES ASSOC | Published : 2011
DOI: 10.2337/db10-0550
Open access
Abstract
OBJECTIVE - Acid sphingomyelinase (ASM) is an important early responder in inflammatory cytokine signaling. The role of ASM in retinal vascular inflammation and vessel loss associated with diabetic retinopathy is not known and represents the goal of this study. RESEARCH DESIGN AND METHODS - Protein and gene expression profiles were determined by quantitative RT-PCR and Western blot. ASM activity was determined using Amplex Red sphingomyelinase assay. Caveolar lipid composition was analyzed by nano-electrospray ionization tandem mass spectrometry. Streptozotocin-induced diabetes and retinal ischemia-reperfusion models were used in in vivo studies. RESULTS - We identify endothelial caveolae-as..
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Awarded by National Eye Institute
Funding Acknowledgements
This work was supported by grants from the Juvenile Diabetes Research Foundation (2-2005-97 to J.V.B.), National Institutes of Health (EY-016077 to J.V.B., RR-025386 to G.E.R. and J.V.B., EY-012601 and EY-007739 to M.B.G., and DK-090730 to M.B.G. and J.V.B.), MEAS (MICL02163 to J.V.B.), and Michigan State University (OVPRGS to J.V.B.).