Cardiolipin Deficiency in Rhodobacter sphaeroides Alters the Lipid Profile of Membranes and of Crystallized Cytochrome Oxidase, but Structure and Function Are Maintained
Xi Zhang, Banita Tamot, Carrie Hiser, Gavin E Reid, Christoph Benning, Shelagh Ferguson-Miller
BIOCHEMISTRY | AMER CHEMICAL SOC | Published : 2011
Many recent studies highlight the importance of lipids in membrane proteins, including in the formation of well-ordered crystals. To examine the effect of changes in one lipid, cardiolipin, on the lipid profile and the production, function, and crystallization of an intrinsic membrane protein, cytochrome c oxidase, we mutated the cardiolipin synthase (cls) gene of Rhodobacter sphaeroides, causing a >90% reduction in cardiolipin content in vivo and selective changes in the abundances of other lipids. Under these conditions, a fully native cytochrome c oxidase (CcO) was produced, as indicated by its activity, spectral properties, and crystal characteristics. Analysis by MALDI tandem mass spect..View full abstract
Awarded by National Institutes of Health
Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
The work was supported by National Institutes of Health Grant R01 GM26916 (S.F.-M.) and the Michigan State University Center of Excellence for the Structural Analysis of Membrane Proteins (C.B., G.E.R., and S.F.-M.).