Journal article
Impaired cellulose synthase guidance leads to stem torsion and twists phyllotactic patterns in arabidopsis
B Landrein, R Lathe, M Bringmann, C Vouillot, A Ivakov, A Boudaoud, S Persson, O Hamant
Current Biology | Published : 2013
Abstract
The parallel alignment of stiff cellulose microfibrils in plant-cell walls mediates anisotropic growth [1, 2]. This is largely controlled by cortical microtubules, which drive the insertion [3, 4] and trajectory of the cellulose synthase (CESA) complex at the plasma membrane [5-7]. The CESA interactive protein 1 (CSI1) acts as a physical linker between CESA and cortical microtubules [8-10]. Here we show that the inflorescence stems of csi1 mutants exhibit subtle right-handed torsion. Because cellulose deposition is largely uncoupled from cortical microtubules in csi1, we hypothesize that strictly transverse deposition of microfibrils in the wild-type is replaced by a helical orientation of u..
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Awarded by Deutsche Forschungsgemeinschaft
Funding Acknowledgements
We are thankful to Vincent Mirabet and Veronique Boltz for help with the analyses and to Platim (UMS 3444 Biosciences Gerland-Lyon Sud) for help with imaging. We are also very grateful to Christy Anna Hipsley for help with microcomputed tomography scanning of Arabidopsis stems. Lastly, we thank Tobias Baskin for insightful comments during the review process. This work was supported by a grant from Agence Nationale de la Recherche (ANR-10-BLAN-1516). M.B. and S.P. were funded through the Max-Planck Gesellschaft and through a Deutsche Forschungsgemeinschaft grant (PE1642/5-1). R.L. was funded through a Deutscher Akademischer Austauschdienst stipend (A/10/75281).