Journal article
Identification of a cellulose synthase-associated protein required for cellulose biosynthesis
Y Gu, N Kaplinsky, M Bringmann, A Cobb, A Carroll, A Sampathkumar, TI Baskin, S Persson, CR Somerville
Proceedings of the National Academy of Sciences of the United States of America | Published : 2010
Abstract
Cellulose synthase-interactive protein 1 (CSI1) was identified in a two-hybrid screen for proteins that interact with cellulose synthase (CESA) isoforms involved in primary plant cell wall synthesis. CSI1 encodes a 2,150-amino acid protein that contains 10 predicted Armadillo repeats and a C2 domain. Mutations in CSI1 cause defective cell elongation in hypocotyls and roots and reduce cellulose content. CSI1 is associated with CESA complexes, and csi1 mutants affect the distribution and movement of CESA complexes in the plasma membrane.
Grants
Awarded by US Department of Energy
Awarded by U.S. Department of Energy (DOE)
Funding Acknowledgements
We thank H. Hofte (INRA, Versailles, France) for providing GFPCESA3 line and S. Li and K. Hematy for helpful discussions. This work was supported in part by an award from The Balzan Foundation, Grants DE-FG02-03ER15421 (to T.I.B.) and DOE-FG02-03ER20133 (to C.R.S.) from the US Department of Energy, and the Energy Biosciences Institute. M.B., A.S., and S.P. were funded through the Max-Planck Gesellschaft.