Journal article
Phosphatidylinositol 4,5-bisphosphate influences PIN polarization by controlling clathrin-mediated membrane trafficking in Arabidopsis
T Ischebeck, S Werner, P Krishnamoorthy, J Lerche, M Meijón, I Stenzel, C Löfke, T Wiessner, YJ Im, IY Perera, T Iven, I Feussner, W Busch, WF Boss, T Teichmann, B Hause, S Persson, I Heilmann
Plant Cell | Published : 2013
Abstract
The functions of the minor phospholipid phosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P2] during vegetative plant growth remain obscure. Here, we targeted two related phosphatidylinositol 4-phosphate 5-kinases (PI4P 5-kinases) PIP5K1 and PIP5K2, which are expressed ubiquitously in Arabidopsis thaliana. A pip5k1 pip5k2 double mutant with reduced PtdIns(4,5)P2 levels showed dwarf stature and phenotypes suggesting defects in auxin distribution. The roots of the pip5k1 pip5k2 double mutant had normal auxin levels but reduced auxin transport and altered distribution. Fluorescence-tagged auxin efflux carriers PIN-FORMED (PIN1)-green fluorescent protein (GFP) and PIN2-GFP displayed abnormal, pa..
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Awarded by European Commission
Funding Acknowledgements
We thank Jiri Friml (Institute of Science and Technology Austria) for Arabidopsis lines expressing fluorescently labeled PIN proteins and for helpful discussion. We also thank Sebastian Bednarek (University of Wisconsin-Madison) for providing Arabidopsis lines expressing fluorescently labeled CLC, Teun Munnik (University of Amsterdam, The Netherlands) for the YFP-PH<INF>PLC delta 1</INF> reporter plants, and Yoshikazu Ohya (University of Tokyo, Japan) for the mss4<SUP>TS</SUP> yeast strains. We thank Ellen Hornung (Georg-August-University Goettingen, Germany) for plasmids. This work was supported by Grants He3424/1 and He3424/2 from the German Research Foundation (Deutsche Forschungsgemeinschaft; to I.H.), by Grant MCB0718452 from the National Science Foundation (to W.F.B. and I.Y.P.), and by a European Molecular Biology long-term fellowship (EMBO-ALTF 299-2010, to T. I.). S.P. was supported by the Max-Planck Society, and P.K. was supported by the European Union Seventh Framework Programme (FP7 2007-2013) under Grant Agreement 263916 (WallTrac, Marie Curie Initial Training Network).