Journal article
POM-POM2/CELLULOSE SYNTHASE INTERACTING1 is essential for the functional association of cellulose synthase and microtubules in Arabidopsis
M Bringmann, E Li, A Sampathkumar, T Kocabek, MT Hauser, S Perssona
Plant Cell | Published : 2012
Abstract
In plants, regulation of cellulose synthesis is fundamental for morphogenesis and plant growth. Cellulose is synthesized at the plasma membrane, and the orientation of synthesis is guided by cortical microtubules; however, the guiding mechanism is currently unknown. We show that the conditional root elongation pom2 mutants are impaired in cell elongation, fertility, and microtubule-related functions. Map-based cloning of the POM-POM2 locus revealed that it is allelic to CELLULOSE SYNTHASE INTERACTING1 (CSI1). Fluorescently tagged POM2/CSI1s associated with both plasma membrane-located cellulose synthases (CESAs) and post-Golgi CESA-containing compartments. Interestingly, while CESA insertion..
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Awarded by Austria Science Foundation (FWF)
Awarded by AKTION Osterreich-Tschechische Republik
Awarded by FWF
Awarded by Deutsche Forschungsgemeinshaft
Awarded by Austrian Science Fund (FWF)
Funding Acknowledgements
We thank Takuji Wada for the pom2-3 allele, Christian Luschnig for the pom2-4 allele, Ryan Gutierrez and Dave Ehrhardt for tdTomato:CESA6, and John Celenza for the cycB1;1:CDB:GUS marker line. We also thank Nicole Schlager, Gernot Resch, Norma Funke, and Anja Froehlich for technical assistance, Thomas Nedoma for the initial mapping, Albert Karsai for T-DNA segregation analysis, and the other members of the lab for advice and patience. The cosmid library was a kind gift of Erwin Grill and Andreas Bachmair. We thank Thomas Herter and Yi Zhang for experimental support. We thank the Kazusa DNA Research Institute (RIKEN) for providing the cDNA clones of POM2. E.L. was supported by the Asia-Europe Forestry Exchange Program and Austria Science Foundation (FWF) Project P13353. T.K. was financed through the AKTION Osterreich-Tschechische Republik Project 21p8. This project was further supported by the FWF Project P14477 to M.-T.H. M.B., A.S., and S.P. were funded through the Max-Planck Gesellschaft and through Deutsche Forschungsgemeinshaft Grant PE1642/5-1. This project was further supported by FWF Projects P14477 and F03707 to M.-T.H.