Evaluation of ELISA coupled with Western blot as a surveillance tool for Trichinella infection in wild boar (Sus scrofa)
Leigh Cuttell, Maria Angeles Gomez-Morales, Beth Cookson, Peter J Adams, Simon A Reid, Paul B Vanderlinde, Louise A Jackson, C Gray, Rebecca J Traub
VETERINARY PARASITOLOGY | ELSEVIER | Published : 2014
Trichinella surveillance in wildlife relies on muscle digestion of large samples which are logistically difficult to store and transport in remote and tropical regions as well as labour-intensive to process. Serological methods such as enzyme-linked immunosorbent assays (ELISAs) offer rapid, cost-effective alternatives for surveillance but should be paired with additional tests because of the high false-positive rates encountered in wildlife. We investigated the utility of ELISAs coupled with Western blot (WB) in providing evidence of Trichinella exposure or infection in wild boar. Serum samples were collected from 673 wild boar from a high- and low-risk region for Trichinella introduction w..View full abstract
This work was funded by the Australian Biosecurity Cooperative Research Centre for Emerging Infectious Diseases (AB-CRC). Sera samples were collected under Animal Ethics Committee approvals CA 2008/02/240 and CA 2011/03/495 by staff from the Northern Australia Quarantine Strategy program of the Australian Government Department of Fisheries, Forestry and Agriculture.