Journal article

Cell-free protein synthesis as a tool to study RXFP3-relaxin-3 protein interactions.

Patrick J Shilling, Ross AD Bathgate, Paul R Gooley

Italian Journal of Anatomy and Embryology | Published : 2013


With the discovery of the relaxin family peptide receptors there is interest in obtaining a clearer understanding of the structure of these proteins and the molecular mechanism of receptor-ligand interaction. As G-protein coupled receptors, obtaining milligram quantities for structural investigations is hampered by the inherent instability of these integral membrane proteins. In the current context, understanding of GPCR structural biology has increased dramatically with crystal structures of several inactive and now active forms solved. In addition, the first nuclear magnetic resonance structure of a GPCR was obtained which is of crucial importance to studying these receptors in a more "bio..

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