A critical re-assessment of DNA repair gene promoter methylation in non-small cell lung carcinoma

H Do; NC Wong; C Murone; T John; B Solomon; PL Mitchell; A Dobrovic

Journal article

A critical re-assessment of DNA repair gene promoter methylation in non-small cell lung carcinoma

H Do, NC Wong, C Murone, T John, B Solomon, PL Mitchell, A Dobrovic

Scientific Reports | Published : 2014

DOI: 10.1038/srep04186

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Abstract

DNA repair genes that have been inactivated by promoter methylation offer potential therapeutic targets either by targeting the specific repair deficiency, or by synthetic lethal approaches. This study evaluated promoter methylation status for eight selected DNA repair genes (ATM, BRCA1, ERCC1, MGMT, MLH1, NEIL1, RAD23B and XPC) in 56 non-small cell lung cancer (NSCLC) tumours and 11 lung cell lines using the methylation-sensitive high resolution melting (MS-HRM) methodology. Frequent methylation in NEIL1 (42%) and infrequent methylation in ERCC1 (2%) and RAD23B (2%) are reported for the first time in NSCLC. MGMT methylation was detected in 13% of the NSCLCs. Contrary to previous studies, me..

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University of Melbourne Researchers

Tom John Author

Benjamin Solomon Author

Grants

Funding Acknowledgements

We would like to thank Thomas Mikeska for critical discussion. This project was supported by funding from Cancer Australia to A. D., the Cancer Council of Victoria to A. D., and the National Health and Medical Research Council of Australia to P. M. and A. D. H. D. is a recipient of Postdoctoral Fellowship from the Cancer Council of Victoria.